![]() ![]() ![]() Question 3 combines these two steps without any hints on the orientation, i.e., it just gives and expects the sequences without explicitly giving the 5' and 3' ends. Question 2 adds the second step, reversing the sequence to give the proper 5'-3' orientation. if the annealing temperature is higher or lower 5 degree compare with Tm, that ratio will be from 20-40 case by case. Each nucleotide is described by a letter (among A, C, G, T, U) and the codon can therefore be described by these 3 letters, but also by the name of the amino acid. ![]() Question 1 simulates the first step, finding the complementary sequence. Tm of primer is temperature when primer can band with DNA at 50 of ratio. What is a codon (Definition) A codon is a sequence of 3 molecules/nucleotides describing an amino acid in the sequencing of DNA or messenger RNA (mRNA) or transfert (tRNA). Give the DNA sequence that will pair with the following stretches of DNA. Line profiling programs indicate that my functions spend a lot of time getting the reverse complements, so I am looking to optimize. It has been determined empirically that there is a 5 (3.4 by Sugimoto et al.) kcal free energy change during the transition from single stranded to B-form DNA. I am writing a python script that requires a reverse complement function to be called on DNA strings of length 1 through around length 30. Codons in an mRNA are read during translation, beginning with a start codon and continuing until a stop codon is reached. One 'start' codon, AUG, marks the beginning of a protein and also encodes the amino acid methionine. Alignments: Pairwise DNA Alignment: Align two DNA sequences and color the resulting global alignment. Reverse Complement: Convert a DNA sequence into its reverse, complement, or reverse-complement. This usually involves reversing the sequence after writing it complementary to the one you are given. We can assume that the concentration of DNA and the concentration of the DNA-primer complex are equal, so this simplifies the equation considerably. Three 'stop' codons mark the end of a protein. DNA Molecular Weight: Calculate the molecular weight of DNA single-stranded sequences are counted with a 5-P. Remember, when writing complementary DNA sequences, you need to write the sequence in the 5' m length of the first sequence, n length. Because of the nature of complementary base pairing, if you know the sequence of one strand of DNA, you can predict the sequence of the strand that will pair with, or "complement" it. Calculate the number of sequence alignments between two DNA or protein sequences. ![]()
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![]() ![]() Louis, Missouri Chinese American News (in Chinese). ![]() ^ "A Sunny Attitude Helps Her Weather All Those J.Lo Jokes".^ a b c d e f g "NBC 5 Hires Nationally Recognized Meteorologist".She and her husband, a Southwest Airlines pilot, have two daughters. Lopez's second degree was a Bachelor of Science in Meteorology from Florida State University. Lopez graduated with a Bachelor of Science in telecommunications from Marquette University. Lopez is a member of the American Meteorological Society (AMS), where she holds the seal of approval in television. On April 20, 2013, Lopez returned to The Weather Channel doing weather updates during taped programming and was an on-camera meteorologist on Weather Center Live. In November 2010, she participated in the Spokes for Hope charity event, where she helped build bicycles for underprivileged children. In 2009, NBC 5 Today, the morning news program for KXAS, and for which Lopez was the meteorologist, won a Lone Star Emmy Award. In June 2008, she became the weekday morning on-camera meteorologist for KXAS-TV in Dallas, Texas after a nationwide search for a replacement for seventeen-year veteran Rebecca Miller. In March 2002, she spoke at the 15th Annual Working Women's Survival Show, discussing her career as a meteorologist. She became the co-host of the PM Edition, which was called Evening Edition at the time. In May 2000, Lopez joined The Weather Channel as on-camera meteorologist. She was also a weather producer and weekend meteorologist at WPTV-TV in West Palm Beach, Florida. In September 1997, Lopez landed her first position as a weekend meteorologist for WTLV-TV in Jacksonville, Florida. She rejoined The Weather Channel in April 2013. She stayed there until 2008, while she started working at KXAS-TV in Dallas, Texas. She began her career in 1997 at WTLV-TV in Jacksonville, Florida, and joined The Weather Channel in 2000. Jennifer Lopez is an American on-camera meteorologist for WSB-TV in Atlanta, Georgia and The Weather Channel. ![]() ![]() Like Shell and Apple, Target has the good fortune to have visual shorthand baked right into its company name. Target has experimented with various relationships between symbol and wordmark over the years More recently, McDonald's has got even more playful in its marketing confidence, such as with these billboards that don't even show the entire logo, and t hese type-only ads. The tagline 'I'm lovin' it' made an appearance in 2003, and went on to replace the company name across many brand touchpoints.ĭecades of ubiquity had given the Golden Arches symbol all the brand equity, confidence and global recognition it needed to stand alone by 2006, without the company name, any accompanying slogans, or any graphic treatments to speak of – just a pared-back, flat-colour 'M' that is unmistakably McDonald's. This approach was refined further in 1975 with the distinctive red background, and many other variants were introduced in different contexts. ![]() In 1968, the Golden Arches adopted their modern-day form – with the company name overlaid at the bottom. ![]() ![]() Once a prominent architectural feature of its restaurants, the Golden Arches first made their way into McDonald's logo in 1961 – before that point, the fast food chain's text-heavy logos incorporated taglines such as 'McDonald's famous barbecue' (1940) and 'McDonald's famous hamburgers. McDonald's distinctive Golden Arches once incorporated the company name – they now have the power to stand alone ![]() ![]() ![]() Once you apply the discount code, your savings should be applied, and you can complete. Screen recordings and screen shots always help a lot. Paste your European Bartender School coupon code and apply it. Please contact us with as much information as possible by sending feedback in Bartender or by emailing. Yes, a license is for use on all the macs you own and are the main user of. It lets you hide any menu bar items you want. Bartender gives you total control over your menu bar items (the icons at the right of your menu bar). If youd like to host it in another install, you can choose the name from the list (if its broadcasting) or type. Bartender 4 is Bartenders’ biggest update yet with support for macOS Big Sur and Apple M1, it also boasts a load of new features and updates to its core abilities making it the best version yet. If you would like to host your license on the current computer, simply choose your computer from the list. On the next screen, specify where youd like to host your license. You will be prompted to enter your previous license details, once submitted and instantly verified you will be able to purchase a Bartender 4 license with a 50% discount. Enter your product key code into the boxes and click Next. How do I buy an upgrade from a previous version?Ĭlick Buy Now, then select Upgrade to Bartender 4. You can then delete any previous versions of Bartender you have and launch Bartender 4. You need to be on macOS Big Sur or Monterey, then you can download Bartender 4 from the website, open the disk image and move Bartender 4 to your applications folder. If you bought Bartender 3 after April 2020 you are entitled to a free upgrade to Bartender 4, Bartender 4 will accept and use your Bartender 3 license. I recently bought Bartender 3 do I need to pay for an upgrade? It has a 4 week trial after this Bartender 4 will be $16, and will have a great discount of 50% for users with a license to any previous version of Bartender. I can provide a version of your Bartender 4 license you could use with Bartender 3 if you need. Because of the massive changes in macOS, Bartender 4 could only be designed to work with the new version of macOS. ![]() Can I use Bartender 4 on macOS Catalina or earlier?īartender 4 is only compatible with macOS Big Sur, Monterey, Ventura and newer. ![]() ![]() Regardless of what industry you’re in, margins are everything. In short, the self storage industry is one of the lowest overhead real estate investments that anyone can make, and therefore, it’s one of the highest margin situations that a landlord can find themselves in. They use automated keypads to provide tenants with entry into their spaces, reducing the need for on-site management.Īnd generally speaking, there isn’t a lot of plumbing or cosmetic maintenance that these properties have to worry about (a good commercial roof and HVAC system for higher premium climate controlled spaces are the main things that a landlord needs). If a tenant stops paying rent, landlords in this industry can recoup some of these costs by selling the assets stored in their facilities.Īnd due to the barebones layouts of these facilities, the risk of property damage inside of each unit is extremely low.įurthermore, self storage facilities have reduced overhead over the years by relying on SEO and digital assistant-like infrastructure to find and book tenants. In general, eviction in the self storage industry is a quick and easy process (especially compared to residential properties). Yes, they still have to pay taxes (that’s unavoidable).īut, tenant issues and overhead are minimized in this space with the use of auctions and automation. You see, investors who own properties in this industry don’t have to deal with the 3 T’, at least, in the traditional sense. Now, if it makes sense for you to invest in physical real estate, when it comes to the commercial property space, one of my absolute favorite industries to invest in and own are self storage facilities. Remember, staying diversified across your investment portfolio is very important… as is staying liquid (it can take weeks, months, or even years to sell large-scale real estate investments). The individuals who make up these teams have decades upon decades of combined experience when it comes to screening investment properties, finding reliable tenants, generating cash flows, and maximizing shareholder value.Īnother problem with investing in physical real estate is that even if you’re someone who is willing to leverage debt to acquire a cash flowing property, there’s a chance that you can’t (or shouldn’t) receive a loan large enough to buy income property(ies). However, simplicity is key to sleeping well at night.ĭividends paid by REITs are truly passive income the only things required to generate these reliable payments are the patience and discipline required to make investments and hold them over the long term.īy owning REITs, you allow professional investment teams and property managers to take on that work (and the headaches that come from the three T’s) for you. ![]() Using leverage to build wealth can be attractive to more risk-tolerant investors. The reason why so many people are REIT investors, rather than physical landlords, is because they’d rather avoid the "three Ts" - toilets, trash, and taxes. You have to deal with tenants, property maintenance, and pay taxes on your assets. What’s more, cash flow coming from real estate is not truly “passive” income.īelieve me, it takes a lot of work to be a landlord. With this in mind, I know many investors who aren’t comfortable with debt-fueled real estate empire plans. Using debt to build a real estate portfolio is one of the lowest risk ways to use leverage to your advantage.īut, debt is still debt… no matter how reliable a property’s cash flow is thought to be. Using debt to accumulate cash flow properties, allowing other people to pay down that debt (tenants) and build your equity, and generate positive cash flows allows real estate investors to build vast fortunes with relatively little cash down. I’ve seen studies showing that real estate is the most common source of value creation for millionaires.Īt the very least, this is likely due to the capital appreciation of the average Joe’s primary residence (assuming that they’re a homeowner).īut many people take this a step further, using investment properties to build wealth and accelerate their journey toward financial freedom. ![]() Real estate is one of the very best ways to build wealth over the long term. Hoarding disorder: An ongoing difficulty throwing away or parting with possessions because you believe that you need to save them. ![]() ![]() ![]() Note: The SELECT statement often includes the ORDER BY clause that puts the results in ascending or descending order. Check the SELECT statement to define the columns to retrieve.Proceed to the WHERE clause to evaluate the criteria for data selection.Evaluate the FROM clause to identify the target table.MySQL works in the following way when executing the SELECT statement containing the WHERE clause: Just select the required data from the lists and enjoy the results. You don’t need to type the columns’ names or remember complex rules. See and visually edit them on the separate tabs of our visual MySQL query tool where you’re drawing your query. There can be a single value, the range between values, the subquery, and more than one condition.Īutomate your work with query criteria. ![]() The WHERE keyword identifies the data selection criteria. SELECT … FROM is the standard statement defining which columns and which table we target. The basic syntax is as follows: SELECT column1, column2. To begin with, let’s take a closer look at using WHERE in the SELECT statement. Using MySQL WHERE clause with comparison operators.In order to make your everyday working routine even more pleasant, it is always better to resort to MySQL profiling and query optimization. It is flexible, allowing the users to specify various conditions for the data selection and combine several conditions in one statement. The MySQL WHERE clause is the handiest means of filtering results. Such clauses determine the selection criteria, so we get only the data we need. When we deal with MySQL, we need conditional clauses for the SELECT statements in the absolute majority of cases. That’s why the current article will deal with this clause and explore its distinguishing features in-depth. The WHERE clause is indispensable for quality work with MySQL databases. ![]() Besides SELECT, the scope of the WHERE clause includes the UPDATE and DELETE statements. Support my content with a Tip.In this article, we are going to talk about the WHERE clause and how to use it in MySQL. ![]() At no additional cost to you, should you make a purchase by clicking through one of them, I will receive a commission. Receive a copy of my ebook, “10 MySQL Tips For Everyone”, absolutely free when you subscribe to the OpenLampTech newsletter.ĭisclosure: Some of this blog post’s services and product links are affiliate links. The Newsletter for PHP and MySQL Developers Find your next digital purchase today!ĭisclosure: Some of this blog post’s services and product links are affiliate links. Mobile wallpapers, digital downloads, photos, development services, and content – all on one Kofi Shop Page.□ 5 Truths I’ve Come To Realize As a Self-taught Developer.Need hosting for your next web application or WordPress site? I highly recommend Hostinger and use them to host my niche bass fishing site.Get a handle on Google Analytics with this course by the folks over at The Content Technologist.Use Coupon Code: KZGZ3WSP when you sign-up today and get 10% off! LoopGenius helps you find customers, spread the word about your side hustle, and turn your idea into a website.My opinions are my own.Ĭheck out this article from the OpenLampTech publication page, Book Recommendation – SQL Antipatterns. Like always, just because you can do something doesn’t mean you should. Your particular goals and needs may vary. Josh Otwell has a passion to grow as a PHP Developer, SQL expert, and technical blogger/writer.ĭisclaimer: The majority of examples in this post, are performed in a personal development/learning workstation environment and should not be considered production quality or ready. ![]() ![]() ![]() There was a manhole in the street, the manhole cover slid away, a vine grew out of it and you could go down the manhole or climb the vine. However, what actually happened somewhat set our future - Robyn never turned the page. I thought it'd be somewhat interactive and you'd turn the page and go to the next interactive stage and tell a story. He's an artist and a musician.' Robyn started on the first page of this book, which held a picture of a fire hydrant. I wanted to do that for software, but I had to convince him. I told him we needed to write a new kind of children's software - like a good quality children's book that adults actually want to read too. A big fan of text adventures such as Zork, Rand was the driving force, having dabbled with games ever since somebody hooked me on a timeshare system and I would steal passwords out of the trash to play the games.Īccording to Rand, the partnership began when he wrote Robyn a letter. Not entirely coincidentally, it was the work of two brothers, Robyn and Rand Miller, who began working on the game in 1991 following a modicum of success with a pioneering series of childrens' titles. The equivalent of Stonehenge (both aesthetically and in terms of pace), Myst involved a bizarre story about two brothers being imprisoned for crimes against literature. An acquired taste it may have been, but the original game has shifted a staggering six million copies, arguably proving the viability of the PC as a gaming medium and helping to establish the healthy scene that we have today. While the gaming world went crazy for Sonic and Mario, it was a brave gamer who would boast of solving lateral thinking puzzles on his dad's home computer'. Users of Myst Masterpiece Edition gave it a rating of 5 out of 5 stars.Arriving at a time when PC games were barely established, Myst rapidly found favour among early adopters, library users and the kind of people who keep the plastic sheeting on the back seat of their cars. Myst Masterpiece Edition runs on the following operating systems: Windows. ![]() It was initially added to our database on. The latest version of Myst Masterpiece Edition is 1.0.0, released on. Myst Masterpiece Edition is a Shareware software in the category Miscellaneous developed by Myst Masterpiece Edition. Overall, Myst Masterpiece Edition is a must-play game for puzzle enthusiasts who crave an immersive experience that requires them to use their logical thinking skills to solve complex challenges and unleash the secrets hidden within the island. As they progress through the game, players learn about the island's history and are tasked with unraveling its mysteries. One of the unique features of Myst Masterpiece Edition is its non-linear gameplay, which allows players to solve the puzzles in their own time and order. They will encounter puzzles of varying difficulty levels that must be solved to progress to different stages of the game. Players must assume the role of the unnamed protagonist and explore the various locations on the island, including hidden underground passages, lakes and forests. The game features stunning graphics and audio, which help create a realistic atmosphere that draws players into the game. ![]() It offers players an immersive puzzle-solving experience that is set on an uninhabited island. Myst Masterpiece Edition is a classic adventure video game that was first released in 1993 and then upgraded in 2000 for modern computers. ![]() ![]() ![]() The track attained similar success internationally, reaching the top 10 in countries such as Australia, Austria, Belgium, Germany, Ireland, Italy, New Zealand and Sweden, as well as number 13 on the Billboard Hot 100. Ellie Goulding Burn. 3:59 Ellie Goulding - Burn (Official Video) 4:02 Burn - Ellie Goulding (Lyrics) 5:11 Ellie Goulding - Burn (Summertime Ball 2014) 4:21 Ellie Goulding - Burn (Live at Capital's Summertime Ball 2023) Capital 5:01 Ellie Goulding - Burn (Live on Good Morning America) + Interview 4:52 Ellie Goulding - Burn (Live From Capital Jingle Bell Ball 2015) 5:03 Ellie Goulding performs \'Burn\' at. It earned Goulding her first number-one single on the UK Singles Chart, selling 116,857 copies in its first week and staying atop the chart for three consecutive weeks. Upon its release, "Burn" was met with a mixed response from music critics, who praised it as "catchy" and noted it as one of Goulding's most radio-friendly songs to date, while others felt it was not memorable. Verse 1 We We dont have to worry bout nothing Cause we got the fire And were burning one hell of a something They Theyre gonna see us from outer space, outer space Light it up Like were the. The song was originally recorded by British singer Leona Lewis for her third studio album, Glassheart (2012), but it was ultimately scrapped. It was released on 5 July 2013 as the lead single from the reissue. FIGURE 8 lyrics by Ellie Goulding: Breathe your smoke into my lungs / In the back of the car with you I stare into the sun / Still not too old to die. ![]() The song was written by Ryan Tedder, Goulding, Greg Kurstin, Noel Zancanella and Brent Kutzle, while production was handled by Kurstin and vocal production was done by Tedder. ![]() "Burn" is a song by English singer Ellie Goulding from Halcyon Days (2013), the reissue of her second studio album, Halcyon (2012). ![]() ![]() ![]() ![]() If you are looking for additional smoothie inspiration, check out one of these smoothie recipe posts below:įeature Image Credit: Photographee. Being a large number of people’s favorite this. This astounding green smoothie is extraordinary compared to other morning drinks. Below we have noted a few additional articles if you are in the market for a new blender, but unsure of where to start! You’ll be thrilled to learn they vary in price, power, and performance-based on its bests uses, so easeful smoothie making is only one quick purchase away! Best Ninja Blender Smoothies Recipes With Directions. See more ideas about blender recipes, recipes, ninja blender recipes. The Ninja Blender might be a hard sell as it’s quite expensive, but there are other blenders on the market that are comparable to these powerful appliances. Explore Jeanine Pecknes board 'Ninja Hot and Cold Blender Recipes', followed by 149 people on Pinterest. Boost the nutrition with flax seeds, protein powder, or chia seeds. Healthy Ninja Foodi Cold And Hot Blender soup recipes that are vegan, cozy, warm, and easy to make Soups are so simple to make. Try adding different fruit like blueberries, or pineapple. We truly hope that this guide helps you become a smoothie ninja. Late-night television infomercials would use these classic words but there is more., well, in this case, there is more.The Ninja Foodi Power Pitcher System is a unique blender/food processor/smoothie maker/dough maker in one tiny 1400 watt device that every kitchen must-have. For a green smoothie, add in a handful of greens and blend with the liquid until smooth before adding the fruit. The best part about smoothie recipes is they are interchangeable, forgiving, and packed full of the things our bodies need. Wrapping UpĪnyone with a blending appliance will be able to easily recreate any of these stand-out Ninja Blender Smoothie Recipes – so feel free to share them among the smoothie lovers in your life. ![]() The 72-ounce main jar can hold enough to serve a crowd. The Ninja Foodi Power Blender Ultimate System is great for making multiple batches of smoothies. Sounds like an experience rather than a smoothie! Get the recipe here. Theres also a personal jar for single-serve smoothies and a Smoothie owl Maker for thicker recipes like nut butter. Served in a bowl, topped with whatever your heart desired, and eaten with a spoon. 12 Low Carb Smoothie Recipes Plus Enter to WIN a Web1 scoop Vanilla whey protein powder 2 fl oz Heavy Whipping Cream 1 tsp Splenda 4 fl oz espresso coffee, brewed and chilled 4 ice cubes Instructions Combine all ingredients in your blender and pulse until slushy. Includes: 1400-peak-watt motor base, 64 oz.* glass pitcher with nonstick coating, tamper, cleaning brush, and 25-recipe inspiration guide (*56 oz.A smoothie with a filling twist – a smoothie bowl incorporates the daily greens and fruits you get from an ordinary smoothie with additional grains, seeds, and fruit. When in use: do not place the appliance near the edge of the countertop provide at least six inches (15.12 pre-set Auto-iQ® smart programs allow you to create smoothies, extractions, frozen drinks, sauces, dips, soups, cocktails, water infusions, ice cream and so much more-all at the touch of a button.64oz.* glass pitcher with nonstick coating and a heated Clean program makes cleanup a breeze.Infuse and unlock flavors with heat to make spa waters, craft cocktails, mixers and more.800-watt precision heating element boils in minutes to cook hearty or smooth soups, simmer flavorful sauces and melt chocolate or cheese into fondue.1400-peak-watt motor base delivers total crushing and extraction for delicious smoothies, resort-style frozen drinks and nutrient extractions.This will reduce wear and tear on the motor and blades, facilitate a. Step 2: Start the blender on its lowest speed and steadily ramp up to its highest speed. Ninja® Foodi™ Cold & Hot Blender -the blender that crushes now brings the heat. THICK PINK PUNCH SMOOTHIE BOWL made with ninjakitchen Foodi Blender SponsoredbyNinja NinjaFoodiBlender NinjaFoodiFamily TooCold vegan Ninja Blender. The Ninja Blender Antioxidant Refresher is very easy to make: Step 1: Place apple, blueberries, celery, red cabbage, watermelon, water, and crushed ice into a blender and secure the lid.Use heat to create concentrates for flavored waters and cocktails. We chatted with Gillean Barkyoumb, R.D., Ninja’s first-ever Smoothie Bowl Sommelier, to learn her best tips for eating healthy when you’re busy, and how to use the Ninja Foodi Smoothie. Cook proteins and veggies for flavorful soups and sauces. Combines high-speed total crushing and extraction with a precision-heat cooking blender to go beyond smoothies and frozen drinks to unlock natural flavors with heat. Meet the Ninja® Foodi™ Cold & Hot Blender. ![]() ![]() ![]() Scroll down to Universal Plug and Play Device Hostĭouble click Universal Plug and Play Device Host If you have a problem with it under XP, try to manually enable UPnP service. Moreover, hit triangle on your video file and you can copy it over to PS3 without hassle!Īccording to Microsoft, it should also work for WMP11 under XP: If you are running Windows Vista you have a very simple way to enable the sharing through Windows Media Player 11. So far we found a reasonably good solution only for Windows OSes, so I'll start with it. DLNA is an open protocol based on UPnP (Universal Plug and Play) and therefore can work on any operating system. Your options for the software naturally depends on your operating system. The latter is most interesting for majority of us, since DLNA certified CE equipment is not that widespread yet. What it means is that you have to have a DLNA server installed, either hardware (like some HDD storages, DVRs or set-top boxes) or as a software on your PC. ![]() (Remote connect to PSP is using proprietary protocols) PS3 takes a role of DLNA Player, not DLNA server, so it can play a content of any DLNA server (source) in you digital home, but can not stream the content to other players within DLNA protocols. ![]() As of FW1.80, DLNA is supported by Playstation 3 entertainment system. OK, since bits of info get burried in a huge FW1.80 thread, I decided to make a new thread where I will update the first post regularly with new info.ĭLNA is an industry standard for media (audio,video, photo) sharing within concept of a digital home and is supported by many hardware and software manufacturers. ![]() |
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